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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGMT Monoclonal antibody (67476-1-Ig)

    WB analysis using 67476-1-Ig (same clone as 67476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67476-1-Ig (MGMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MGMT Monoclonal antibody (67476-1-Ig)

    WB analysis using 67476-1-Ig (same clone as 67476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67476-1-Ig (MGMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MGMT Monoclonal antibody (67476-1-Ig)

    WB analysis of HeLa using 67476-1-Ig (same clone as 67476-1-PBS)

    WB result of MGMT antibody (67476-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGMT transfected HeLa cells. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MGMT Monoclonal antibody (67476-1-Ig)

    IF Staining of HepG2 using 67476-1-Ig (same clone as 67476-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGMT antibody (67476-1-Ig, Clone: 1H2C9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using MGMT Monoclonal antibody (67476-1-Ig)

    FC experiment of Jurkat using 67476-1-Ig (same clone as 67476-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MGMT (67476-1-Ig, Clone:1H2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MGMT Monoclonal antibody, PBS Only

MGMT Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67476-1-PBS
Clone No.1H2C9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1H2C9, 6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67476-1-PBS targets MGMT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29936

Product name: Recombinant human MGMT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-207 aa of BC000824

Sequence: MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN

相同性解析による交差性が予測される生物種
Full Name O-6-methylguanine-DNA methyltransferase
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000824
Gene Symbol MGMT
Gene ID (NCBI) 4255
RRIDAB_2882703
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP16455
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]

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