Validation Data Gallery

Filter: AllWBIHC

WB analysis using 67468-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67468-1-Ig
WB result of MGME1 antibody (67468-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGME1 transfected HeLa cells.
WB analysis of HL-60 using 67468-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 67468-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67468-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67468-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67468-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67468-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67468-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

MGME1 Monoclonal antibody

MGME1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67468-1-Ig

Clone No.2C4G1

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

C20orf72, 2C4G1, DDK1, EC:3.1.-.-, Mitochondrial genome maintenance exonuclease 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HL-60 cells, HepG2 cells, LNCaP cells, A549 cells, Jurkat cells, K-562 cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IP	See 1 publications below

Product Information

67468-1-Ig targets MGME1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag19644 Product name: Recombinant human MGME1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC016869 Sequence: MKMKLFQTICRQLRSSKFSVESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCGPVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTEKKKNQNIQKPEYSE 相同性解析による交差性が予測される生物種
Full Name	chromosome 20 open reading frame 72
Calculated molecular weight	344 aa, 39 kDa
Observed molecular weight	36-39 kDa
GenBank accession number	BC016869
Gene Symbol	MGME1
Gene ID (NCBI)	92667
RRID	AB_2882697
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9BQP7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for MGME1 antibody 67468-1-Ig	Download protocol
WB protocol for MGME1 antibody 67468-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Biochem Biophys Res Commun Unspliced XBP1 enhences metabolic reprogramming in colorectal cancer cells by interfering with the mitochondrial localization of MGME1 Authors - Jiandong Zhang View Article
human	WB	Aging (Albany NY) MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma Authors - Feng Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67468-1-Ig

WB analysis of HeLa using 67468-1-Ig

WB analysis of HL-60 using 67468-1-Ig

IHC staining of human lung cancer using 67468-1-Ig

IHC staining of human lung cancer using 67468-1-Ig

IHC staining of human colon cancer using 67468-1-Ig

IHC staining of human colon cancer using 67468-1-Ig

IHC staining of human colon cancer using 67468-1-Ig

IHC staining of human colon cancer using 67468-1-Ig

MGME1 Monoclonal antibody

MGME1 Monoclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Unspliced XBP1 enhences metabolic reprogramming in colorectal cancer cells by interfering with the mitochondrial localization of MGME1

MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma

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