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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MGLL Polyclonal antibody (14986-1-AP)

    WB analysis of HepG2 using 14986-1-AP (same clone as 14986-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MGLL Polyclonal antibody (14986-1-AP)

    WB analysis using 14986-1-AP (same clone as 14986-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using MGLL Polyclonal antibody (14986-1-AP)

    IP experiment of mouse liver using 14986-1-AP (same clone as 14986-1-PBS)

    IP result of anti-MGLL (IP:14986-1-AP, 4ug; Detection:14986-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MGLL Polyclonal antibody (14986-1-PBS)

    IHC staining of human liver cancer using 14986-1-PBS

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MGLL Polyclonal antibody (14986-1-PBS)

    IHC staining of human liver cancer using 14986-1-PBS

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MGLL Polyclonal antibody (14986-1-AP)

    IHC staining of human liver using 14986-1-AP (same clone as 14986-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MGLL Polyclonal antibody (14986-1-AP)

    IHC staining of human kidney using 14986-1-AP (same clone as 14986-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MGLL Polyclonal antibody, PBS Only

MGLL Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14986-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14986-1-PBS targets MGLL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6970

Product name: Recombinant human MGLL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC000551

Sequence: METGPEDPSSMPEESSPRRTPQSIPYQDLPHLVNADGQYLFCRYWKPTGTPKALIFVSHGAGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASPP

相同性解析による交差性が予測される生物種
Full Name monoglyceride lipase
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC000551
Gene Symbol MGLL
Gene ID (NCBI) 11343
RRIDAB_2143189
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA0A0C4DFN3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

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