Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle |
| Positive IHC detected in | human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29885-1-AP targets MGC33407/ACTL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31805 Product name: Recombinant human MGC33407 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-172 aa of BC024028 Sequence: MDASRPKSSESQSSLEAPRPGPNPSPNVVNKPLQRDSPGMVADRLPPKTGAVVIDMGTGTCKVGFAGQASPTYTVATILGCQPKKPATSGQSGLQTFIGEAARVLPELTLVQPLRSGIVVDWDAAELIWRHLLEHDLRVATHDHPLLFSDPPFSPATNREKLVEVAFESLRS 相同性解析による交差性が予測される生物種 |
| Full Name | hypothetical protein MGC33407 |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC024028 |
| Gene Symbol | ACTL9 |
| Gene ID (NCBI) | 284382 |
| RRID | AB_2923617 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TC94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MGC33407/ACTL9 antibody 29885-1-AP | Download protocol |
| IHC protocol for MGC33407/ACTL9 antibody 29885-1-AP | Download protocol |
| WB protocol for MGC33407/ACTL9 antibody 29885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |