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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    WB analysis using 29885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of human liver using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of human liver using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of mouse testis using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of mouse testis using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using ACTL9 Polyclonal antibody (29885-1-AP)

    IF Staining of mouse testis using 29885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using ACTL9 Polyclonal antibody (29885-1-AP)

    IF Staining of mouse testis using 29885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ACTL9 Polyclonal antibody

ACTL9 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29885-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29885-1-AP targets ACTL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31805

Product name: Recombinant human MGC33407 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-172 aa of BC024028

Sequence: MDASRPKSSESQSSLEAPRPGPNPSPNVVNKPLQRDSPGMVADRLPPKTGAVVIDMGTGTCKVGFAGQASPTYTVATILGCQPKKPATSGQSGLQTFIGEAARVLPELTLVQPLRSGIVVDWDAAELIWRHLLEHDLRVATHDHPLLFSDPPFSPATNREKLVEVAFESLRS

相同性解析による交差性が予測される生物種
Full Name hypothetical protein MGC33407
Observed molecular weight45 kDa
GenBank accession numberBC024028
Gene Symbol ACTL9
Gene ID (NCBI) 284382
RRIDAB_2923617
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.

Protocols

Product Specific Protocols
IF protocol for ACTL9 antibody 29885-1-APDownload protocol
IHC protocol for ACTL9 antibody 29885-1-APDownload protocol
WB protocol for ACTL9 antibody 29885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.

Authors - Chuan Wang
View Article
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