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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of SH-SY5Y using 16993-1-AP (same clone as 16993-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of MCF-7 using 16993-1-AP (same clone as 16993-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of C6 using 16993-1-AP (same clone as 16993-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of Jurkat using 16993-1-AP (same clone as 16993-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MGAT5B Polyclonal antibody (16993-1-AP)

    IP experiment of Jurkat using 16993-1-AP (same clone as 16993-1-PBS)

    IP result of anti-MGAT5B (IP:16993-1-AP, 4ug; Detection:16993-1-AP 1:700) with Jurkat cells lysate 3600ug. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    IF Staining of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16993-1-AP (MGAT5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

MGAT5B Polyclonal antibody, PBS Only

MGAT5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16993-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16993-1-PBS targets MGAT5B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10678

Product name: Recombinant human MGAT5B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-394 aa of BC062354

Sequence: MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGVLRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILRHSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPPLPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQRQRRLRSSQEQARCGVMGACEKMLLEGDVASNTLLIEKPSKKETEKCPKNLVT

相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B
Calculated molecular weight 394 aa, 44 kDa, 89 kDa
Observed molecular weight 89-100 kDa
GenBank accession numberBC062354
Gene Symbol MGAT5B
Gene ID (NCBI) 146664
RRIDAB_10858919
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3V5L5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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