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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGAT4B Polyclonal antibody (30718-1-AP)

    WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MGAT4B Polyclonal antibody (30718-1-AP)

    WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MGAT4B Polyclonal antibody (30718-1-AP)

    IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MGAT4B Polyclonal antibody (30718-1-AP)

    IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MGAT4B Polyclonal antibody (30718-1-AP)

    IHC staining of mouse kidney using 30718-1-AP (same clone as 30718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MGAT4B Polyclonal antibody (30718-1-AP)

    IF Staining of U2OS using 30718-1-AP (same clone as 30718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MGAT4B antibody (30718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

MGAT4B Polyclonal antibody, PBS Only

MGAT4B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30718-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30718-1-PBS targets MGAT4B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33769

Product name: Recombinant human MGAT4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 306-446 aa of BC009464

Sequence: KTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD

相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Calculated molecular weight 548 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC009464
Gene Symbol MGAT4B
Gene ID (NCBI) 11282
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ53
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).

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