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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MGAT4A Polyclonal antibody

MGAT4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25109-1-AP

Synonyms

GlcNAc T IVa, GNT IV, GNT IVA, MGAT4A

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 25109-1-AP

    WB analysis of HeLa using 25109-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25109-1-AP (MGAT4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25109-1-AP

    IHC staining of human small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25109-1-AP (MGAT4A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 25109-1-AP

    IHC staining of human small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25109-1-AP (MGAT4A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25109-1-AP is MGAT4A Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGAT4A fusion protein Ag18956 相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Calculated molecular weight 535 aa, 62 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC127107
Gene symbol MGAT4A
Gene ID (NCBI) 11320
RRIDAB_2879901
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MGAT4A antibody 25109-1-APDownload protocol
IHC protocol for MGAT4A antibody 25109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Biochim Biophys Acta Mol Cell Res

N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility

Authors - Depeng Yang
View Article