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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using MGAT3 Polyclonal antibody (17869-1-AP)

    WB analysis of COLO 320 using 17869-1-AP (same clone as 17869-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using MGAT3 Polyclonal antibody (17869-1-AP)

    WB analysis of Caco-2 using 17869-1-AP (same clone as 17869-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGAT3 Polyclonal antibody (17869-1-AP)

    IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGAT3 Polyclonal antibody (17869-1-AP)

    IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MGAT3 Polyclonal antibody, PBS Only

MGAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17869-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17869-1-PBS targets MGAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12352

Product name: Recombinant human MGAT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 184-533 aa of BC075026

Sequence: VPTVVQYSNLPTKERLVPREVPRRVINAINVNHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTVDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRGLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDRFHYLLDNPYQEPRSTAAGGWRHRGPEGRPPARGKLDEAEV

相同性解析による交差性が予測される生物種
Full Name mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
Calculated molecular weight 533 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC075026
Gene Symbol MGAT3
Gene ID (NCBI) 4248
RRIDAB_10597697
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09327
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.

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