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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGAT2 Recombinant antibody (85933-1-RR)

    WB analysis using 85933-1-RR (same clone as 85933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85933-1-RR (MGAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using MGAT2 Recombinant antibody (85933-1-RR)

    IF Staining of Raji using 85933-1-RR (same clone as 85933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using MGAT2 antibody (85933-1-RR, Clone: 250147B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85933-1-PBS in a different storage buffer formulation.

MGAT2 Recombinant monoclonal antibody, PBS Only

MGAT2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85933-1-PBS
Clone No.250147B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Beta-1,2-N-acetylglucosaminyltransferase II, CDGS2, EC:2.4.1.143, GlcNAc T II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85933-1-PBS targets MGAT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8423

Product name: Recombinant human MGAT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 101-447 aa of BC006390

Sequence: DQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ

相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Calculated molecular weight 447 aa, 52 kDa
Observed molecular weight55-60 kDa
GenBank accession numberBC006390
Gene Symbol MGAT2
Gene ID (NCBI) 4247
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ10469
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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