Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Y79 using 15103-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15103-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15103-1-AP (MGAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of mouse liver using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse heart using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of mouse heart using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15103-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 15103-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MGAT1 antibody (15103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MGAT1 Polyclonal antibody

MGAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15103-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GlcNAc-T I, GLCNAC TI, GlcNAc T I, GGNT1, EC:2.4.1.101

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Y79 cells, HepG2 cells
Positive IHC detected in	human liver cancer tissue, human heart tissue, mouse heart tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15103-1-AP targets MGAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7446 Product name: Recombinant human MGAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-445 aa of BC003575 Sequence: VIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPPTWEGYDPSWN 相同性解析による交差性が予測される生物種
Full Name	mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Calculated molecular weight	39 kDa
Observed molecular weight	51-55 kDa
GenBank accession number	BC003575
Gene Symbol	MGAT1
Gene ID (NCBI)	4245
RRID	AB_2297618
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26572
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for MGAT1 antibody 15103-1-AP	Download protocol
IHC protocol for MGAT1 antibody 15103-1-AP	Download protocol
WB protocol for MGAT1 antibody 15103-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Arthritis Rheum Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage. Authors - Urita Atsushi A View Article
	WB	Cell Mol Life Sci N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion. Authors - Laura Hobohm View Article
mouse	WB	FASEB J Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy. Authors - Andrew R Ednie View Article
human, rat	WB	Schizophr Res Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia. Authors - Jordyn M Kippe View Article
mouse	WB	Liver Transpl Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis. Authors - Kim H H Liss View Article
rat	WB,IF	Sci Rep Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats. Authors - Kaimei Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Y79 using 15103-1-AP

WB analysis of HepG2 using 15103-1-AP

IHC staining of human liver cancer using 15103-1-AP

IHC staining of mouse liver using 15103-1-AP

IHC staining of mouse liver using 15103-1-AP

IHC staining of mouse heart using 15103-1-AP

IHC staining of mouse heart using 15103-1-AP

IHC staining of human heart using 15103-1-AP

IHC staining of human heart using 15103-1-AP

IF Staining of HeLa using 15103-1-AP

MGAT1 Polyclonal antibody

MGAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage.

N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.

Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.

Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia.

Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis.

Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats.