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  • Featured Product
  • KD/KO Validated

MFN2 Monoclonal antibody, PBS Only

MFN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5F3B3

Cat no : 67487-1-PBS

Synonyms

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MFN2 Monoclonal antibody (67487-1-Ig)

    WB analysis of HeLa using 67487-1-Ig (same clone as 67487-1-PBS)

    WB result of MFN2 antibody (67487-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells. This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MFN2 Monoclonal antibody (67487-1-Ig)

    WB analysis using 67487-1-Ig (same clone as 67487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67487-1-Ig (MFN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MFN2 Monoclonal antibody (67487-1-Ig)

    IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MFN2 Monoclonal antibody (67487-1-Ig)

    IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MFN2 Monoclonal antibody (67487-1-Ig)

    IF Staining of HepG2 using 67487-1-Ig (same clone as 67487-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN2 antibody (67487-1-Ig, Clone: 5F3B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67487-1-PBS targets MFN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MFN2 fusion protein Ag29873 相同性解析による交差性が予測される生物種
Full Name mitofusin 2
Calculated molecular weight 757 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC017061
Gene symbol MFN2
Gene ID (NCBI) 9927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.