MFI2 Polyclonal antibody
MFI2 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 10428-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Transfected |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
10428-1-AP targets MFI2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MFI2 fusion protein Ag0700 相同性解析による交差性が予測される生物種 |
| Full Name | antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 82 kDa |
| GenBank accession number | BC001875 |
| Gene symbol | MFI2 |
| Gene ID (NCBI) | 4241 |
| RRID | AB_2142592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological Marker of colorectal cancer (PMID: 25216327).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MFI2 antibody 10428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling | ||
Reprod Sci MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration | ||
Front Immunol Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis |
