Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, C2C12 cells, H9C2 cells, mouse heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87972-1-RR targets MFF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag9990 Product name: Recombinant human MFF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-238 aa of BC000797 Sequence: MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial fission factor |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 27-38 kDa |
| GenBank accession number | BC000797 |
| Gene Symbol | MFF |
| Gene ID (NCBI) | 56947 |
| RRID | AB_3745841 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9GZY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mitochondrial Fission Factor (MFF) is a critical regulator of organelle division, specifically playing a central role in the fission of mitochondria and peroxisomes. DRP1, MiD, and MFF form a trimeric complex in which MiD proteins facilitate the MFF-DRP1 interaction. Moreover, MiD51 can inhibit MFF-induced activation of DRP1 GTPase activity. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.(PMID: 39365854, PMID: 37291333)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MFF antibody 87972-1-RR | Download protocol |
| WB protocol for MFF antibody 87972-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |