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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MFAP4 Recombinant monoclonal antibody (88535-1-RR)

    WB analysis using 88535-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88535-1-RR (MFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MFAP4 Recombinant monoclonal antibody (88535-1-RR)

    WB analysis using 88535-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88535-1-RR (MFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using MFAP4 Recombinant monoclonal antibody (88535-1-RR)

    IHC staining of human colon using 88535-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MFAP4 Recombinant monoclonal antibody (88535-1-RR)

    IHC staining of human colon using 88535-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using MFAP4 Recombinant monoclonal antibody (88535-1-RR)

    IHC staining of human lung using 88535-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using MFAP4 Recombinant monoclonal antibody (88535-1-RR)

    IHC staining of human lung using 88535-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MFAP4 Recombinant monoclonal antibody

MFAP4 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 88535-1-RR
Clone No.261177D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Microfibril-associated glycoprotein 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue
Positive IHC detected inhuman colon tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88535-1-RR targets MFAP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11920

Product name: Recombinant human MFAP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC062415

Sequence: MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGVGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA

相同性解析による交差性が予測される生物種
Full Name microfibrillar-associated protein 4
Calculated molecular weight 255 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC062415
Gene Symbol MFAP4
Gene ID (NCBI) 4239
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP55083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

Protocols

Product Specific Protocols
IHC protocol for MFAP4 antibody 88535-1-RRDownload protocol
WB protocol for MFAP4 antibody 88535-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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