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Validation Data Gallery

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  • Western Blot (WB) analysis of C2C12 cells using MEX3B Polyclonal antibody (30185-1-AP)

    WB analysis of C2C12 using 30185-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 30185-1-AP (MEX3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using MEX3B Polyclonal antibody (30185-1-AP)

    WB analysis of SGC-7901 using 30185-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30185-1-AP (MEX3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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MEX3B Polyclonal antibody

MEX3B Polyclonal Antibody for WB, ELISA
Cat No. 30185-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MEX 3B, RING finger and KH domain-containing protein 3, RKHD3, RNA-binding protein MEX3B, RNF195

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC2C12 cells, SGC-7901 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30185-1-AP targets MEX3B in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEX3B fusion protein Ag32899 相同性解析による交差性が予測される生物種
Full Name mex-3 homolog B (C. elegans)
Calculated molecular weight 569 aa, 59 kDa
Observed molecular weight60-70 kDa
GenBank accession numberBC036211
Gene Symbol MEX3B
Gene ID (NCBI) 84206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZN04
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human MEX3B (hMEX3B) protein is an RNA-binding protein that contains two KH domains at the N-terminus and a RING domain at its C-terminus, which has the activity of E3 ubiquitin ligase and is essential for RNA degradation.

Protocols

Product Specific Protocols
WB protocol for MEX3B antibody 30185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols