Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | C2C12 cells, SGC-7901 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30185-1-AP targets MEX3B in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32899 Product name: Recombinant human MEX3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-458 aa of BC036211 Sequence: YFGGGTSSSAAATQRLADYSPPSPALSFAHNGNNNNNGNGYTYTAGGEASVPSPDGCPELQPTFDPAPAPPPGAPLIWAQFERSPGGGPAAPVSSSCSSSASSSASSSSVVFPGSGASAPSNANLGLLVHRRLHPGTSCPRLSPPLHMAPGAGEHHLAR 相同性解析による交差性が予測される生物種 |
| Full Name | mex-3 homolog B (C. elegans) |
| Calculated molecular weight | 569 aa, 59 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC036211 |
| Gene Symbol | MEX3B |
| Gene ID (NCBI) | 84206 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN04 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human MEX3B (hMEX3B) protein is an RNA-binding protein that contains two KH domains at the N-terminus and a RING domain at its C-terminus, which has the activity of E3 ubiquitin ligase and is essential for RNA degradation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MEX3B antibody 30185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |