Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
15120-1-AP targets METTL9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | METTL9 fusion protein Ag7191 相同性解析による交差性が予測される生物種 |
| Full Name | methyltransferase like 9 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC000195 |
| Gene Symbol | METTL9 |
| Gene ID (NCBI) | 51108 |
| RRID | AB_2878109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H1A3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for METTL9 antibody 15120-1-AP | Download protocol |
| IHC protocol for METTL9 antibody 15120-1-AP | Download protocol |
| IF protocol for METTL9 antibody 15120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Rep Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers.
| ||
Cell Death Discov METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition |