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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL8 Polyclonal antibody (31173-1-AP)

    WB analysis using 31173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31173-1-AP (METTL8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using METTL8 Polyclonal antibody (31173-1-AP)

    IF Staining of A431 using 31173-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using METTL8 antibody (31173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

METTL8 Polyclonal antibody

METTL8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31173-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

methyltransferase like 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 205 cells, HCT 116 cells, LoVo cells, SW480 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31173-1-AP targets METTL8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL8 fusion protein Ag34222 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 8
Calculated molecular weight33 kDa
Observed molecular weight33~40 kDa
GenBank accession numberBC025250
Gene Symbol METTL8
Gene ID (NCBI) 79828
RRIDAB_3669881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H825
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL8 (methyltransferase-like protein 8) is an m3C methyltransferase that is best known for its mitochondrial role in installing m3C32 on mt-tRNAThr/Ser(UCN). METTL8 has proved to be a transcriptional target of STAT3 that mediates STAT3's functions in mESCs (PMID: 29706498). METTL8 has multiple splicing isoforms and can undergo sumoylation and associate with nuclear RNA-binding proteins to regulate R-loop formation on ribosomal DNA gene (presumably via its methyltransferase activity on m3C) (PMID: 38744809).

Protocols

Product Specific Protocols
WB protocol for METTL8 antibody 31173-1-APDownload protocol
IF protocol for METTL8 antibody 31173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols