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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL7A Monoclonal antibody (67905-1-Ig)

    WB analysis using 67905-1-Ig (same clone as 67905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using METTL7A Monoclonal antibody (67905-1-Ig)

    WB analysis using 67905-1-Ig (same clone as 67905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using METTL7A Monoclonal antibody (67905-1-Ig)

    WB analysis of HepG2 using 67905-1-Ig (same clone as 67905-1-PBS)

    WB result of METTL7A antibody (67905-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells. This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    WB analysis of mouse lung using 67905-1-Ig (same clone as 67905-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 67905-1-Ig (METTL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    IHC staining of human thyroid cancer using 67905-1-Ig (same clone as 67905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    IHC staining of human thyroid cancer using 67905-1-Ig (same clone as 67905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    IHC staining of human liver cancer using 67905-1-Ig (same clone as 67905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    IHC staining of human liver cancer using 67905-1-Ig (same clone as 67905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    IHC staining of human liver cancer using 67905-1-Ig (same clone as 67905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Monoclonal antibody (67905-1-Ig)

    IHC staining of human liver cancer using 67905-1-Ig (same clone as 67905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67905-1-Ig (METTL7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67905-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

METTL7A Monoclonal antibody, PBS Only

METTL7A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67905-1-PBS
Clone No.1A9B4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

AAM B, EC:2.1.1.348, EC:2.1.1.9, methyltransferase like 7A, Methyltransferase-like protein 7A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67905-1-PBS targets METTL7A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29986

Product name: Recombinant human METTL7A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-244 aa of BC008180

Sequence: MELTIFILRLAIYILTFPLYLLNFLGLWSWICKKWFPYFLVRFTVIYNEQMASKKRELFSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFLIKSIAENRHLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEHVAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALERASFSKLKLQHIQAPLSWELVRPHIYGYAVK

相同性解析による交差性が予測される生物種
Full Name methyltransferase like 7A
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC008180
Gene Symbol METTL7A
Gene ID (NCBI) 25840
RRIDAB_2918661
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H8H3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.

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