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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL7A Polyclonal antibody (17092-1-AP)

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using METTL7A Polyclonal antibody (17092-1-AP)

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using METTL7A Polyclonal antibody (17092-1-AP)

    WB analysis of HepG2 using 17092-1-AP

    WB result of METTL7A antibody (17092-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

METTL7A Polyclonal antibody

METTL7A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17092-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse lung tissue, rat lung tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17092-1-AP targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10013

Product name: Recombinant human METTL7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC008180

Sequence: MELTIFILRLAIYILTFPLYLLNFLGLWSWICKKWFPYFLVRFTVIYNEQMASKKRELFSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFLIKSIAENRHLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEHVAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALERASFSKLKLQHIQAPLSWELVRPHIYGYAVK

相同性解析による交差性が予測される生物種
Full Name methyltransferase like 7A
Calculated molecular weight 28 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC008180
Gene Symbol METTL7A
Gene ID (NCBI) 25840
RRIDAB_2878348
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for METTL7A antibody 17092-1-APDownload protocol
WB protocol for METTL7A antibody 17092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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