Validation Data Gallery
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Tested Applications
| Positive WB detected in | Ramos cells, THP-1 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00070 targets METTL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | methyltransferase like 5 |
| Calculated molecular weight | 209 aa, 24 kDa |
| Observed molecular weight | 24-27 kDa |
| GenBank accession number | BC000921 |
| Gene Symbol | METTL5 |
| Gene ID (NCBI) | 29081 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q9NRN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL5 antibody RMX00070 | Download protocol |
| IHC protocol for METTL5 antibody RMX00070 | Download protocol |
| WB protocol for METTL5 antibody RMX00070 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |