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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL5 Recombinant antibody (85682-5-RR)

    WB analysis using 85682-5-RR (same clone as 85682-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85682-5-RR (METTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85682-5-PBS in a different storage buffer formulation.

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METTL5 Recombinant antibody, PBS Only

METTL5 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85682-5-PBS
Clone No.243081C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85682-5-PBS targets METTL5 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL5 fusion protein Ag10214 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 5
Calculated molecular weight 209 aa, 24 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberBC000921
Gene Symbol METTL5
Gene ID (NCBI) 29081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRN9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.