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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8H1

Cat no : CL594-67733

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

Validation Data Gallery

Applications:
  • IF Staining of mouse testis using CL594-67733

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

  • IF Staining of mouse testis using CL594-67733

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

  • IF Staining of mouse testis using CL594-67733

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67733

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67733

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67733

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL594-67733

    FC experiment of HEK-293 using CL594-67733

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human METTL3 (CL594-67733, Clone:2D8H1) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inmouse testis tissue
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67733-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67733 targets METTL3 in IF, FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2920176
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 METTL3 antibody CL594-67733Download protocol
FC protocol for CL594 METTL3 antibody CL594-67733Download protocol
Standard Protocols
Click here to view our Standard Protocols