Validation Data Gallery
Tested Applications
| Positive WB detected in | 5637 cells |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24379-1-AP targets METTL13 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19355 Product name: Recombinant human METTL13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 541-642 aa of BC029083 Sequence: QSDRMKVHIADGLDYIASLAGGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRRI 相同性解析による交差性が予測される生物種 |
| Full Name | methyltransferase like 13 |
| Calculated molecular weight | 699 aa, 79 kDa |
| Observed molecular weight | 72-78 kDa |
| GenBank accession number | BC029083 |
| Gene Symbol | METTL13 |
| Gene ID (NCBI) | 51603 |
| RRID | AB_3742110 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N6R0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein methyltransferase like 13 (METTL13), also called FEAT, is coded by gene METTL13, which is located at chromosome 1q24.3. Purified from rat livers in 2011 by a Japanese researcher, METTL13 was found to be abnormally overexpressed in several human cancers including lung cancer and to drive tumorigenesis in transgenic mice. A study indicated that METTL13 inhibits apoptosis of lung, breast and liver cancer cells and miR-16 can repress the expression of METTL13 by binding to its 3′-untranslated region. (PMID: 33985542)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL13 antibody 24379-1-AP | Download protocol |
| WB protocol for METTL13 antibody 24379-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |