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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL11A Polyclonal antibody (17184-1-AP)

    WB analysis using 17184-1-AP (same clone as 17184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17184-1-AP (METTL11A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using METTL11A Polyclonal antibody (17184-1-AP)

    IHC staining of human ovary cancer using 17184-1-AP (same clone as 17184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17184-1-AP (METTL11A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17184-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using METTL11A Polyclonal antibody (17184-1-AP)

    IF Staining of HeLa using 17184-1-AP (same clone as 17184-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using METTL11A antibody (17184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17184-1-PBS in a different storage buffer formulation.

METTL11A Polyclonal antibody, PBS Only

METTL11A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17184-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AD 003, AD-003, Alpha N-terminal protein methyltransferase 1A, C9orf32, EC:2.1.1.244

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17184-1-PBS targets METTL11A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10967

Product name: Recombinant human METTL11A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-223 aa of BC033234

Sequence: MTSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR

相同性解析による交差性が予測される生物種
Full Name methyltransferase like 11A
Calculated molecular weight 223 aa, 25 kDa
Observed molecular weight16 kDa, 26 kDa
GenBank accession numberBC033234
Gene Symbol METTL11A
Gene ID (NCBI) 28989
RRIDAB_2142020
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV86
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

METTL11A, also known as NTMT1 (Nα-trimethyllysine hydrolase), is an enzyme that belongs to the methyltransferase-like (METTL) protein family. It plays a crucial role in epigenetic processes, particularly in the methylation of proteins, which can influence gene expression and other cellular functions. METTL11A is regulated by other members of the METTL family, such as METTL11B and METTL13, which can either activate or inhibit its function.

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