Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, DU 145 cells, Daudi cells, Raji cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33763-1-AP targets METTL10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37000 Product name: Recombinant human METTL10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC026167 Sequence: MSSGADGGGGAAVAARSDKGSPGEDGFVPSALGTREHWDAVYERELQTFREYGDTGEIWFGEESMNRLIRWMQKHKIPLDASVLDIGTGNGVFLVELAKFGFSNITGIDYSPSAIQLSGSIIEKEGLSNIKLKVEDFLNLSTQLSGFHICIDKGTFDAISLNPDNAIEKRKQYVKSLSRVLKVKGFFSNNVM 相同性解析による交差性が予測される生物種 |
| Full Name | methyltransferase like 10 |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC026167 |
| Gene Symbol | METTL10 |
| Gene ID (NCBI) | 399818 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5JPI9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Methyltransferase-like 10 (METTL10) is a lysine trimethyltransferase targeting eukaryotic translation elongation factor 1A1 (eEF1A1), which is well preserved from simple eukaryotes such as Saccharomyces cerevisiae to mammals such as humans and mice. Recently, METTL10was identified as an estimated glomerular filtration rate (eGFR)-associated gene through a transethnic genome-wide association study of eGFR. (PMID: 39774961)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL10 antibody 33763-1-AP | Download protocol |
| IHC protocol for METTL10 antibody 33763-1-AP | Download protocol |
| WB protocol for METTL10 antibody 33763-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |