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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL1 Monoclonal antibody (68501-1-Ig)

    WB analysis using 68501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68501-1-Ig (METTL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using METTL1 Monoclonal antibody (68501-1-Ig)

    WB analysis using 68501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68501-1-Ig (METTL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

METTL1 Monoclonal antibody

METTL1 Monoclonal Antibody for WB, ELISA
Cat No. 68501-1-Ig
Clone No.2C3G7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, ELISA

Synonyms

C12orf1, FLJ95748, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68501-1-Ig targets METTL1 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7055

Product name: Recombinant human METTL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-276 aa of BC000550

Sequence: MAAETRNVAGAEAPPPQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQTSLPGH

相同性解析による交差性が予測される生物種
Full Name methyltransferase like 1
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC000550
Gene Symbol METTL1
Gene ID (NCBI) 4234
RRIDAB_3085212
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UBP6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

Protocols

Product Specific Protocols
WB protocol for METTL1 antibody 68501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols