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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METRN Polyclonal antibody (16327-1-AP)

    WB analysis using 16327-1-AP (same clone as 16327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16327-1-AP (METRN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using METRN Polyclonal antibody (16327-1-AP)

    IHC staining of mouse brain using 16327-1-AP (same clone as 16327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using METRN Polyclonal antibody (16327-1-AP)

    IHC staining of mouse brain using 16327-1-AP (same clone as 16327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using METRN Polyclonal antibody (16327-1-AP)

    IHC staining of mouse heart using 16327-1-AP (same clone as 16327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using METRN Polyclonal antibody (16327-1-AP)

    IHC staining of mouse heart using 16327-1-AP (same clone as 16327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16327-1-PBS in a different storage buffer formulation.

METRN Polyclonal antibody, PBS Only

METRN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16327-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

C16orf23, Meteorin, RJD6

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16327-1-PBS targets METRN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9414

Product name: Recombinant human METRN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-293 aa of BC000662

Sequence: YSEERCSWRGSGLTQEPGSVGQLALACAEGAVEWLYPAGALRLTLGGPDPRARPGIACLRPVRPFAGAQVFAERAGGALELLLAEGPGPAGGRCVRWGPRERRALFLQATPHQDISRRVAAFRFELREDGRPELPPQAHGLGVDGACRPCSDAELLLAACTSDFVIHGIIHGVTHDVELQESVITVVAARVLRQTPPLFQAGRSGDQGLTSIRTPLRCGVHPGPGTFLFMGWSRFGEARLGCAPRFQEFRRAYEAARAAHLHPCEVALH

相同性解析による交差性が予測される生物種
Full Name meteorin, glial cell differentiation regulator
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC000662
Gene Symbol METRN
Gene ID (NCBI) 79006
RRIDAB_3669238
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJH8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Meteorin, also named as METRN and C16orf23, is a secret protein which plays important roles in both glial cell differentiation and axonal network formation during neurogenesis.

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