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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METAP1 Polyclonal antibody (28702-1-AP)

    WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using METAP1 Polyclonal antibody (28702-1-AP)

    WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using METAP1 Polyclonal antibody (28702-1-AP)

    IHC staining of human ovary cancer using 28702-1-AP (same clone as 28702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28702-1-AP (METAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using METAP1 Polyclonal antibody (28702-1-AP)

    IF Staining of HEK-293 using 28702-1-AP (same clone as 28702-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using METAP1 antibody (28702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

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METAP1 Polyclonal antibody, PBS Only

METAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28702-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Peptidase M 1, MetAP 1, MAP 1, KIAA0094

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28702-1-PBS targets METAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METAP1 fusion protein Ag29828 相同性解析による交差性が予測される生物種
Full Name methionyl aminopeptidase 1
Calculated molecular weight 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberNM_015143
Gene Symbol METAP1
Gene ID (NCBI) 23173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53582
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

METAP1 (methionyl aminopeptidase 1), also known as MAP1A or MetAP1A, is expected to be located in cytosol and widely expressed in esophagus and testis. The calculated molecular weight of METAP1 is 43 kDa. METAP1 is expected to have aminopeptidase activity and metallo-exopeptidase activity. It is also involved in platelet aggregation. At the same time, HsMetAP1 participates in co-translation to remove N- terminal methionine from newborn protein. Studies have shown that when HsMetAP1 is knocked down by gene-specific siRNA, cells show slow progress in G(2)/M phase, which is similar to cells treated with MetAP1 inhibitors. Importantly, MetAP1 inhibitors can induce apoptosis of leukemia cell lines, which may be the result of their interference with G(2)/M checkpoint. Therefore, it is necessary for normal cell cycle, especially in G(2)/M phase of cell cycle, and it can be used as a promising target for discovering and developing new anticancer drugs (PMID: 17114291).