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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEI4 Polyclonal antibody (29892-1-AP)

    WB analysis using 29892-1-AP (same clone as 29892-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 29892-1-AP (MEI4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MEI4 Polyclonal antibody (29892-1-AP)

    IHC staining of mouse testis using 29892-1-AP (same clone as 29892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MEI4 Polyclonal antibody (29892-1-AP)

    IHC staining of mouse testis using 29892-1-AP (same clone as 29892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using MEI4 Polyclonal antibody (29892-1-AP)

    IF Staining of mouse testis using 29892-1-AP (same clone as 29892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MEI4 antibody (29892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29892-1-PBS in a different storage buffer formulation.

MEI4 Polyclonal antibody, PBS Only

MEI4 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29892-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Meiosis-specific protein MEI4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29892-1-PBS targets MEI4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31859

Product name: Recombinant human MEI4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of NM_001322247.1

Sequence: MDVQKWYLRTSKLALALAIIRSKPADKSSREYTEHLAMLLSEEQSKWRSKVEILEAEVMQLRQKLLVSRLCSGSFKSGYVSSQLEAQEPKSSESTLTSMEDSGCDLSNEQRTESSDLSQHFVESCTPTHFPPLPLVKRPCAILQNPLSSHMQFLQYLLELKNLTESGNLKRDLTHFEKDSSTVSDS*

相同性解析による交差性が予測される生物種
Full Name Meiosis-specific protein MEI4
Calculated molecular weight44 kDa
Observed molecular weight45 kDa
GenBank accession numberNM_001322247.1
Gene Symbol MEI4
Gene ID (NCBI) 101928601
RRIDAB_3086182
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA8MW99
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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