• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MEF2D Recombinant antibody (85801-5-RR)

    WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEF2D Recombinant antibody (85801-5-RR)

    WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEF2D Recombinant antibody (85801-5-RR)

    WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MEF2D Recombinant antibody (85801-5-RR)

    IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MEF2D Recombinant antibody (85801-5-RR)

    IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEF2D Recombinant antibody (85801-5-RR)

    IF Staining of HeLa using 85801-5-RR (same clone as 85801-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (85801-5-RR, Clone: 250068B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85801-5-RR

    Affinity and Kinetic Characterization of 85801-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85801-5-RR against Human MEF2D were performed. The affinity constant is 1.99 nM.

［PR］論文掲載5,600報突破！GFP免疫沈降用抗体「GFP-Trap®」を無料体験

MEF2D Recombinant antibody, PBS Only

MEF2D Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85801-5-PBS
Clone No.250068B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Myocyte-specific enhancer factor 2D

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85801-5-PBS targets MEF2D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MEF2D fusion protein Ag5700 相同性解析による交差性が予測される生物種
Full Name myocyte enhancer factor 2D
Calculated molecular weight 56 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC040949
Gene Symbol MEF2D
Gene ID (NCBI) 4209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14814
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.