Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of C2C12 using 14353-1-AP
C2C12 cell were subjected to SDS PAGE followed by western blot with 14353-1-AP (MEF2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14353-1-AP
WB result of MEF2D antibody (14353-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEF2D transfected HeLa cells.
WB analysis of HeLa using 14353-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14353-1-AP (MEF2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 14353-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14353-1-AP (MEF2D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14353-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14353-1-AP (MEF2D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14353-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MEF2D antibody (14353-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of A431 using 14353-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using MEF2D antibody (14353-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

MEF2D Polyclonal antibody

MEF2D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14353-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

Myocyte-specific enhancer factor 2D

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	C2C12 cell, HeLa cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 17 publications below
IHC	See 2 publications below
IF	See 2 publications below
ChIP	See 1 publications below

Product Information

14353-1-AP targets MEF2D in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5700 Product name: Recombinant human MEF2D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-514 aa of BC040949 Sequence: SLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSANGACPSPVGNGYVSARASPGLLPVANGNSLNKVIPAKSPPPPTHSTQLGAPSRKPDLRVITSQAGKGLMHHLNNAQRLGVSQSTHSLTTPVVSVATPSLLSQGLPFSSMPTAYNTDYQLTSAELSSLPAFSSPGGLSLGNVTAWQQPQQPQQPQQPQPPQQQPPQPQQPQPQQPQQPQQPPQQQSHLVPVSLSNLIPGSPLPHVGAALTVTTHPHISIKSEPVSPSRERSPAPPPPAVFPAARPEPGDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPAPEPEAEGSAVKRMRLDTWTLK 相同性解析による交差性が予測される生物種
Full Name	myocyte enhancer factor 2D
Calculated molecular weight	56 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC040949
Gene Symbol	MEF2D
Gene ID (NCBI)	4209
RRID	AB_2878046
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14814
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.

Protocols

Product Specific Protocols
IF protocol for MEF2D antibody 14353-1-AP	Download protocol
IHC protocol for MEF2D antibody 14353-1-AP	Download protocol
WB protocol for MEF2D antibody 14353-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodeling. Authors - Huimin Li View Article
human	WB	Autophagy MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis Authors - Yuji Shiozaki View Article
human, mouse	WB	Nat Commun Targeting chaperone-mediated autophagy inhibits properties of glioblastoma stem cells and restores anti-tumor immunity. Authors - Yonghua Li View Article
mouse	WB,IF	Nat Commun Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis Authors - Mónika Gönczi View Article
human	WB	Int J Biol Sci Core transcriptional regulatory circuitry molecule ZNF217 promotes AML cell proliferation by up-regulating MYB Authors - Bi Zhou KD Validated View Article
human	WB	Acta Pharmacol Sin Identification of AKOS, a Chikungunya virus inhibitor, as a USP14 inhibitor for colorectal cancer treatment. Authors - Bin Lu View Article

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Validation Data Gallery

WB analysis of C2C12 using 14353-1-AP

WB analysis of HeLa using 14353-1-AP

WB analysis of HeLa using 14353-1-AP

IHC staining of mouse brain using 14353-1-AP

IHC staining of mouse brain using 14353-1-AP

IF Staining of HepG2 using 14353-1-AP

IF Staining of A431 using 14353-1-AP

MEF2D Polyclonal antibody

MEF2D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodeling.

MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis

Targeting chaperone-mediated autophagy inhibits properties of glioblastoma stem cells and restores anti-tumor immunity.

Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis

Core transcriptional regulatory circuitry molecule ZNF217 promotes AML cell proliferation by up-regulating MYB

Identification of AKOS, a Chikungunya virus inhibitor, as a USP14 inhibitor for colorectal cancer treatment.

Able^™

Validation Data Gallery

WB analysis of C2C12 using 14353-1-AP

WB analysis of HeLa using 14353-1-AP

WB analysis of HeLa using 14353-1-AP

IHC staining of mouse brain using 14353-1-AP

IHC staining of mouse brain using 14353-1-AP

IF Staining of HepG2 using 14353-1-AP

IF Staining of A431 using 14353-1-AP

MEF2D Polyclonal antibody

MEF2D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodeling.

MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis

Targeting chaperone-mediated autophagy inhibits properties of glioblastoma stem cells and restores anti-tumor immunity.

Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis

Core transcriptional regulatory circuitry molecule ZNF217 promotes AML cell proliferation by up-regulating MYB

Identification of AKOS, a Chikungunya virus inhibitor, as a USP14 inhibitor for colorectal cancer treatment.

Able™

Able^™