Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue, HEK-293 cells |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 4 publications below |
Product Information
16787-1-AP targets MED30 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10221 Product name: Recombinant human MED30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC008226 Sequence: MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGTYQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 30 |
| Calculated molecular weight | 178 aa, 20 kDa |
| Observed molecular weight | 20-25 kDa |
| GenBank accession number | BC008226 |
| Gene Symbol | MED30 |
| Gene ID (NCBI) | 90390 |
| RRID | AB_2142439 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MED30 antibody 16787-1-AP | Download protocol |
| WB protocol for MED30 antibody 16787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Autoimmun Immune complexome analysis of a rich variety of serum immune complexes identifies disease-characteristic immune complex antigens in systemic sclerosis | ||
Anticancer Res The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
| ||
Ann Diagn Pathol The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.
| ||
Nat Genet Interaction between MED12 and ΔNp63 activates basal identity in pancreatic ductal adenocarcinoma |
