Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, SMMC-7721 cells, HuH-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 13 publications below |
| IHC | See 8 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
| ChIP | See 5 publications below |
Product Information
11566-1-AP targets MED15 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2143 Product name: Recombinant human MED15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC017110 Sequence: DPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHSMAVVSTATPQTQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQ 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 15 |
| Calculated molecular weight | 788 aa, 87 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC017110 |
| Gene Symbol | MED15 |
| Gene ID (NCBI) | 51586 |
| RRID | AB_2141975 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RN5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MED15 antibody 11566-1-AP | Download protocol |
| IHC protocol for MED15 antibody 11566-1-AP | Download protocol |
| IP protocol for MED15 antibody 11566-1-AP | Download protocol |
| WB protocol for MED15 antibody 11566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy | ||
Mol Cell Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. | ||
Mol Cell Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors | ||
Nucleic Acids Res PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts | ||
Cell Death Discov MED15 is upregulated by HIF-2α and promotes proliferation and metastasis in clear cell renal cell carcinoma via activation of SREBP-dependent fatty acid synthesis |