Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20047-1-AP targets MED12L in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 12-like |
| Calculated molecular weight | 240 kDa |
| Observed molecular weight | 260-280 kDa |
| GenBank accession number | NM_053002 |
| Gene Symbol | MED12L |
| Gene ID (NCBI) | 116931 |
| RRID | AB_10734594 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86YW9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The antibody can recognize all the isoform of MED12L. The antibody has no cross reaction to MED12.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED12L antibody 20047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
