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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MECR Recombinant monoclonal antibody (84729-4-RR)

    WB analysis using 84729-4-RR (same clone as 84729-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84729-4-RR (MECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MECR Recombinant monoclonal antibody (84729-4-RR)

    WB analysis of HeLa using 84729-4-RR (same clone as 84729-4-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84729-4-RR (MECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MECR Recombinant monoclonal antibody (84729-4-RR)

    IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MECR Recombinant monoclonal antibody (84729-4-RR)

    IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MECR Recombinant monoclonal antibody (84729-4-RR)

    IF Staining of NIH/3T3 using 84729-4-RR (same clone as 84729-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MECR antibody (84729-4-RR, Clone: 242151C5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using MECR Recombinant monoclonal antibody (84729-4-RR)

    IF Staining of C2C12 using 84729-4-RR (same clone as 84729-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MECR antibody (84729-4-RR, Clone: 242151C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84729-4-RR

    Affinity and Kinetic Characterization of 84729-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84729-4-RR against Mouse MECR were performed. The affinity constant is below 1 pM.

Mouse MECR Recombinant monoclonal antibody, PBS Only

MECR Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84729-4-PBS
Clone No.242151C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242151C5, 2-enoyl thioester reductase, EC:1.3.1.104, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, Nrbf1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84729-4-PBS targets MECR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0465

Product name: Recombinant mouse Mecr protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 117-373 aa of BC003864

Sequence: SSVSALKPGDWVIPANAGLGTWRTEAVFSEEALIGIPKDIPLQSAATLGVNPCTAYRMLVDFEQLQPGDSVIQNASNSGVGQAVIQIASALRLKTINVVRDRPDIKKLTDRLKDLGADYVLTEEELRMPETKTIFKDLPLPRLALNCVGGKSSTELLRHLAPGGTMVTYGGMAKQPVTASVSLLIFKDLKLRGFWLSQWKKNHSPDEFKELILTLCNLIRQGRLTAPSCSEVPLQGYQQALEASMKPFVSSKQILTM

相同性解析による交差性が予測される生物種
Full Name mitochondrial trans-2-enoyl-CoA reductase
Calculated molecular weight 40 kDa
Observed molecular weight37-40 kDa
GenBank accession numberBC003864
Gene Symbol MECR
Gene ID (NCBI) 26922
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9DCS3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial 2-enoyl-acyl-carrier protein reductase (MECR) is an enzyme in the mitochondrial fatty acid synthase (mtFAS) pathway. MECR is involved in the synthesis of lipoic acid and is required for mitochondrial respiratory competence. MECR is highly expressed in skeletal and heart muscles and exhibits lower expression in placenta, liver, kidney, and pancreas. It is weakly expressed or not expressed in the lung (PMID: 32440681, PMID: 37734847, PMID: 38296034).

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