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  • Featured Product
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ME2 Monoclonal antibody

ME2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6E6E2

Cat no : 67457-1-Ig

Synonyms

Malic enzyme 2, ME2, NAD ME

Validation Data Gallery

Applications:
  • WB analysis using 67457-1-Ig

    WB analysis using 67457-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67457-1-Ig

    WB analysis of HeLa using 67457-1-Ig

    WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells.

  • WB analysis of human peripheral blood platelets using 67457-1-Ig

    WB analysis of human peripheral blood platelets using 67457-1-Ig

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67457-1-Ig

    WB analysis of HeLa using 67457-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67457-1-Ig

    WB analysis of HepG2 using 67457-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67457-1-Ig

    IHC staining of human liver cancer using 67457-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67457-1-Ig

    IHC staining of human liver cancer using 67457-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67457-1-Ig

    IHC staining of human liver cancer using 67457-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67457-1-Ig

    IHC staining of human liver cancer using 67457-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, human peripheral blood platelets, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67457-1-Ig targets ME2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ME2 fusion protein Ag20741 相同性解析による交差性が予測される生物種
Full Name malic enzyme 2, NAD(+)-dependent, mitochondrial
Calculated molecular weight 65 kDa
Observed molecular weight 54 kDa, 65 kDa
GenBank accession numberBC000147
Gene symbol ME2
Gene ID (NCBI) 4200
RRIDAB_2882689
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

Protocols

Product Specific Protocols
WB protocol for ME2 antibody 67457-1-IgDownload protocol
IHC protocol for ME2 antibody 67457-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Nat Commun

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis

Authors - Taiqi Chen
View Article