ME1 Monoclonal antibody
ME1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, rabbit, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G9G9
Cat no : 68117-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, pig brain tissue, HCT 116 cells, LNCaP cells, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Sample-dependent, check data in validation data gallery |
Product Information
68117-1-Ig targets ME1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
Tested Reactivity | Human, mouse, rat, rabbit, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ME1 fusion protein Ag9916 相同性解析による交差性が予測される生物種 |
Full Name | malic enzyme 1, NADP(+)-dependent, cytosolic |
Calculated molecular weight | 572 aa, 64 kDa |
Observed molecular weight | 55-64 kDa |
GenBank accession number | BC025246 |
Gene symbol | ME1 |
Gene ID (NCBI) | 4199 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH.
Protocols
Product Specific Protocols | |
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WB protocol for ME1 antibody 68117-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |