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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using MDM1-Specific Polyclonal antibody (16171-1-AP)

    WB analysis of human brain using 16171-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16171-1-AP (MDM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MDM1-Specific Polyclonal antibody (16171-1-AP)

    WB analysis of human brain using 16171-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16171-1-AP (MDM1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MDM1-Specific Polyclonal antibody

MDM1-Specific Polyclonal Antibody for WB, ELISA
Cat No. 16171-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

MDM1, Nuclear protein MDM1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16171-1-AP targets MDM1-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name Mdm1 nuclear protein homolog (mouse)
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberNM_020128
Gene Symbol MDM1
Gene ID (NCBI) 56890
RRIDAB_1851359
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)

Protocols

Product Specific Protocols
WB protocol for MDM1-Specific antibody 16171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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