Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15462-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15462-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 15462-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 15462-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15462-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 15462-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 15462-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15462-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 15462-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15462-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15462-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 15462-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MDH2 antibody (15462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MDH2 Polyclonal antibody

MDH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15462-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:1.1.1.37, MDH, MDH 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse lung tissue, rat lung tissue, HepG2 cells, L02 cells
Positive IHC detected in	human liver cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 23 publications below
IP	See 1 publications below

Product Information

15462-1-AP targets MDH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, caenorhabditis elegans
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7750 Product name: Recombinant human MDH2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-338 aa of BC001917 Sequence: MLSALARPVSAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK 相同性解析による交差性が予測される生物種
Full Name	malate dehydrogenase 2, NAD (mitochondrial)
Calculated molecular weight	36 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC001917
Gene Symbol	MDH2
Gene ID (NCBI)	4191
RRID	AB_2878143
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P40926
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).

Protocols

Product Specific Protocols
IF protocol for MDH2 antibody 15462-1-AP	Download protocol
IHC protocol for MDH2 antibody 15462-1-AP	Download protocol
WB protocol for MDH2 antibody 15462-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma Authors - Qifan Hu View Article
human	WB	Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Authors - Xiao-Hui Wang View Article
human	WB	J Exp Med Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth. Authors - Miao Liu View Article
mouse	WB	Aging Dis Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging Authors - Minghao Yuan View Article
mouse	WB	iScience The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways. Authors - Tirthankar Sinha View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure. Authors - Qianqian Guo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15462-1-AP

WB analysis using 15462-1-AP

WB analysis using 15462-1-AP

WB analysis using 15462-1-AP

WB analysis of mouse lung using 15462-1-AP

WB analysis of rat lung using 15462-1-AP

IHC staining of human liver cancer using 15462-1-AP

IHC staining of human liver cancer using 15462-1-AP

IHC staining of human colon using 15462-1-AP

IHC staining of human lung cancer using 15462-1-AP

IHC staining of human lung cancer using 15462-1-AP

IF Staining of U2OS using 15462-1-AP

MDH2 Polyclonal antibody

MDH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.