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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of Kasumi-1 cells using MDFIC Polyclonal antibody (31740-1-AP)

    IP experiment of Kasumi-1 using 31740-1-AP

    IP result of anti-MDFIC (IP:31740-1-AP, 4ug; Detection:31740-1-AP 1:500) with Kasumi-1 cells lysate 1320 ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MDFIC Polyclonal antibody (31740-1-AP)

    IF Staining of HEK-293 using 31740-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MDFIC antibody (31740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MDFIC Polyclonal antibody

MDFIC Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 31740-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

HIC, I-mfa domain-containing protein, LMPHM12, MyoD family inhibitor domain-containing protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inKasumi-1 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31740-1-AP targets MDFIC in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDFIC fusion protein Ag35437 相同性解析による交差性が予測される生物種
Full Name MyoD family inhibitor domain containing
Calculated molecular weight26 kDa
Observed molecular weight32 kDa
GenBank accession numberNM_001166345
Gene Symbol MDFIC
Gene ID (NCBI) 29969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9P1T7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDFIC encodes the MyoD family inhibitor domain containing protein, a 246-amino acid protein documented to regulate the activity of transcription factors including the glucocorticoid recep tor (GR), HAND1, and T cell factor/lymphoid enhancer factor (TCF/LEF) family members. The C-terminal region of MDFIC harbors a domain extremely rich in cysteine residues, reported to mediate protein-protein interactions between MDFIC and transcription factor componentry (PMID: 35235341).

Protocols

Product Specific Protocols
IF protocol for MDFIC antibody 31740-1-APDownload protocol
IP protocol for MDFIC antibody 31740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols