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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using M-CSF Recombinant antibody (84481-4-RR)

    WB analysis using 84481-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84481-4-RR (MCSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84481-4-RR

    Affinity and Kinetic Characterization of 84481-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84481-4-RR against Human M-CSF were performed. The affinity constant is 11.4 nM.

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M-CSF Recombinant antibody

M-CSF Recombinant Antibody for WB, ELISA
Cat No. 84481-4-RR
Clone No.241743C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

M CSF, Lanimostim, CSF-1, CSF1, CSF 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84481-4-RR targets M-CSF in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 1 (macrophage)
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC021117
Gene Symbol M-CSF
Gene ID (NCBI) 1435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09603
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Protocols

Product Specific Protocols
WB protocol for M-CSF antibody 84481-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols