Validation Data Gallery

Filter: AllWBIHC

WB analysis of Jurkat using 11362-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11362-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11362-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11362-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 11362-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 11362-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 11362-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 11362-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 11362-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11362-1-AP (MCRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

MCRS1 Polyclonal antibody

MCRS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11362-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Synonyms

58 kDa microspherule protein, ICP22BP, INO80 complex subunit J, INO80Q, MCRS1, MCRS2, microspherule protein 1, MSP58, P78

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, human brain tissue, human liver tissue, human spleen tissue, mouse spleen tissue, mouse uterus tissue, rat spleen tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IF	See 2 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

Product Information

11362-1-AP targets MCRS1 in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1904 Product name: Recombinant human MCRS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-462 aa of BC011794 Sequence: MDKDSQGLLDSSLMASGTASRSEDEESLAGQKRASSQALGTIPKRRSSSRFIKRKKFDDELVESSLAKSSTRAKGASGVEPGRCSGSEPSSSEKKKVSKAPSTPVPPSPAPAPGLTKRVKKSKQPLQVTKDLGRWKPADDLLLINAVLQTNDLTSVHLGVKFSCRFTLREVQERWYALLYDPVISKLACQAMRQLHPEAIAAIQSKALFSKAEEQLLSKVGSTSQPTLETFQDLLHRHPDAFYLARTAKALQAHWQLMKQYYLLEDQTVQPLPKGDQVLNFSDAEDLIDDSKLKDMRDEVLEHELMVADRRQKREIRQLEQELHKWQVLVDSITGMSSPDFDNQTLAVLRGRMVRYLMRSREITLGRATKDNQIDVDLSLEGPAWKISRKQGVIKLKNNGDFFIANEGRRPIYIDGRPVLCGSKWRLSNNSVVEIASLRFVFLINQDLIALIRAEAAKITPQ 相同性解析による交差性が予測される生物種
Full Name	microspherule protein 1
Calculated molecular weight	51 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC011794
Gene Symbol	MCRS1
Gene ID (NCBI)	10445
RRID	AB_2143116
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96EZ8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.

Protocols

Product Specific Protocols
IHC protocol for MCRS1 antibody 11362-1-AP	Download protocol
WB protocol for MCRS1 antibody 11362-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria. Authors - Aindrila Chatterjee View Article
mouse	WB	Elife MOF-associated complexes ensure stem cell identity and Xist repression. Authors - Tomasz Chelmicki View Article
mouse	WB,IF,CoIP	EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis Authors - Jia-Qian Ju KO Validated View Article
human	IF	Sci Rep MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. Authors - Si-Hyung Lee View Article
human	WB	J Biol Chem Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Authors - Chen Lu L View Article
human	WB	Mol Biol Cell Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment. Authors - Hongdan Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11362-1-AP

WB analysis of HeLa using 11362-1-AP

WB analysis of human brain using 11362-1-AP

WB analysis of human liver using 11362-1-AP

WB analysis of mouse spleen using 11362-1-AP

WB analysis of rat spleen using 11362-1-AP

WB analysis of mouse uterus using 11362-1-AP

WB analysis of human spleen using 11362-1-AP

IHC staining of mouse testis using 11362-1-AP

MCRS1 Polyclonal antibody

MCRS1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.

MOF-associated complexes ensure stem cell identity and Xist repression.

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.

Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling.

Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.