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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® MCP-1/CCL2 Multi-Recombinant antibody (RMX00085)

    WB analysis using RMX00085

    Non-treated, LPS and Brefeldin A treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with RMX00085 (MCP-1/CCL2 antibody) at dilution of 1:1800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using Multi-rAb® MCP-1/CCL2 Multi-Recombinant antibody (RMX00085)

    FC experiment of RAW 264.7 using RMX00085

    1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.2 ug Mouse MCP-1/Ccl2 Multi-Recombinant Antibody (RMX00085) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Multi-rAb® MCP-1/CCL2 Multi-Recombinant antibody

MCP-1/CCL2 Multi-Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. RMX00085

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLPS and Brefeldin A treated RAW 264.7 cells
Positive FC (Intra) detected inLPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00085 targets MCP-1/CCL2 in WB, FC (Intra), ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
Observed molecular weight16-20 kDa
GenBank accession numberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP10148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Monocyte chemotactic protein 1 (MCP-1), also known as CCL2, is chemotactic for monocyte/macrophage, B cell, and T cell, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Protocols

Product Specific Protocols
FC protocol for MCP-1/CCL2 antibody RMX00085Download protocol
WB protocol for MCP-1/CCL2 antibody RMX00085Download protocol
Standard Protocols
Click here to view our Standard Protocols
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