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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM5 Monoclonal antibody (67049-1-Ig)

    WB analysis using 67049-1-Ig (same clone as 67049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using MCM5 Monoclonal antibody (67049-1-Ig)

    WB analysis of A375 using 67049-1-Ig (same clone as 67049-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MCM5 Monoclonal antibody (67049-1-Ig)

    WB analysis of HeLa using 67049-1-Ig (same clone as 67049-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MCM5 Monoclonal antibody (67049-1-Ig)

    IF Staining of HepG2 using 67049-1-Ig (same clone as 67049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67049-1-Ig (MCM5 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

MCM5 Monoclonal antibody, PBS Only

MCM5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67049-1-PBS
Clone No.3B7F8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CDC46, CDC46 homolog, MCM5, P1 CDC46

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67049-1-PBS targets MCM5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28704

Product name: Recombinant human MCM5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 388-734 aa of BC000142

Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 5
Calculated molecular weight 82 kDa
Observed molecular weight 82-88 kDa
GenBank accession numberBC000142
Gene Symbol MCM5
Gene ID (NCBI) 4174
RRIDAB_2882362
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP33992
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

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