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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM2 Monoclonal antibody (66204-1-Ig)

    WB analysis using 66204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MCM2 Monoclonal antibody (66204-1-Ig)

    WB analysis of HEK-293 using 66204-1-Ig

    WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of A431 cells using MCM2 Monoclonal antibody (66204-1-Ig)

    WB analysis of A431 using 66204-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MCM2 Monoclonal antibody (66204-1-Ig)

    WB analysis of K-562 using 66204-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MCM2 Monoclonal antibody (66204-1-Ig)

    WB analysis of HEK-293 using 66204-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using MCM2 Monoclonal antibody (66204-1-Ig)

    WB analysis of 4T1 using 66204-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Human tonsillitis tissue using MCM2 Monoclonal antibody (66204-1-Ig)

    IHC staining of Human tonsillitis using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66204-1-Ig (MCM2 antibody) at dilution of 1:2000 (under 20x lens).

  • Immunohistochemistry (IHC) staining of Human tonsillitis tissue using MCM2 Monoclonal antibody (66204-1-Ig)

    IHC staining of Human tonsillitis using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66204-1-Ig (MCM2 antibody) at dilution of 1:2000 (under 20x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MCM2 Monoclonal antibody (66204-1-Ig)

    IHC staining of human prostate cancer using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MCM2 Monoclonal antibody (66204-1-Ig)

    IHC staining of human prostate cancer using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

MCM2 Monoclonal antibody

MCM2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66204-1-Ig
Clone No.1F4D10
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1F4D10, BM28, CCNL1, CDCL1, EC:3.6.4.12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells, A431 cells, 4T1 cells, A549 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inHuman tonsillitis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66204-1-Ig targets MCM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM2 fusion protein Ag0798 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 2
Calculated molecular weight 102 kDa
Observed molecular weight 120-125 kDa
GenBank accession numberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
RRIDAB_2881595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MCM2 antibody 66204-1-IgDownload protocol
IHC protocol for MCM2 antibody 66204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Death Discov

MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling

Authors - Xin Zhou
View Article