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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCEE Recombinant monoclonal antibody (85948-1-RR)

    WB analysis using 85948-1-RR (same clone as 85948-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85948-1-RR (MCEE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85948-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using MCEE Recombinant monoclonal antibody (85948-1-RR)

    WB analysis of rat liver using 85948-1-RR (same clone as 85948-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 85948-1-RR (MCEE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85948-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85948-1-RR

    Affinity and Kinetic Characterization of 85948-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85948-1-RR against Human MCEE were performed. The affinity constant is 0.126 nM.

MCEE Recombinant monoclonal antibody, PBS Only

MCEE Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85948-1-PBS
Clone No.250393G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

DL methylmalonyl CoA racemase, DL-methylmalonyl-CoA racemase, EC:5.1.99.1, GLOD2, MCE

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85948-1-PBS targets MCEE in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9665

Product name: Recombinant human MCEE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC020825

Sequence: MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGLDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA

相同性解析による交差性が予測される生物種
Full Name methylmalonyl CoA epimerase
Calculated molecular weight 176 aa, 19 kDa
Observed molecular weight17 kDa
GenBank accession numberBC020825
Gene Symbol MCEE
Gene ID (NCBI) 84693
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96PE7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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