Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HL-60 cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15536-1-AP targets MCAT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7867 Product name: Recombinant human MCAT protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-390 aa of BC042195 Sequence: MSVRVARVAWVRGLGASYRRGASSFPVPPPGAQGVAELLRDATGAEEEAPWAATERRMPGQCSVLLFPGQGSQVVGMGRGLLNYPRVRELYAAARRVLGYDLLELSLHGPQETLDRTVHCQPAIFVASLAAVEKLHHLQPSVIENCVAAAGFSVGEFAALVFAGAMEFAEGLYAVKIRAEAMQEASEAVPSGMLSVLGQPQSKFNFACLEAREHCKSLGIENPVCEVSNYLFPDCRVISGHQEALRFLQKNSSKFHFRRTRMLPVSGAFHTRLMEPAVEPLTQALKAVDIKKPLVSVYSNVHGHRYRHPGHIHKLLAQQLVSPVKWEQTMHAIYERKKGRGFPQTFEVGPGRQLGAILKSCNMQAWKSYSAVDVLQTLEHVDLDPQEPPR 相同性解析による交差性が予測される生物種 |
| Full Name | malonyl CoA:ACP acyltransferase (mitochondrial) |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC042195 |
| Gene Symbol | MCAT |
| Gene ID (NCBI) | 27349 |
| RRID | AB_3669211 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Malonyl-CoA-acyl carrier protein transacylase (MCAT), also known as MT, is an enzyme involved in mitochondrial fatty acid synthesis (mtFAS) and catalyzes the transfer of the malonyl moiety of malonyl-CoA to the mitochondrial acyl carrier protein (ACP) (PMID: 36881526). It has four coding exons and is transcribed into two isoforms by the alternative splicing of exon 3 (PMID: 33918393).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MCAT antibody 15536-1-AP | Download protocol |
| WB protocol for MCAT antibody 15536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |