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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using MBOAT7 Recombinant monoclonal antibody (83546-3-RR)

    IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MBOAT7 Recombinant monoclonal antibody (83546-3-RR)

    IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using MBOAT7 Recombinant monoclonal antibody (83546-3-RR)

    IF Staining of HuH-7 using 83546-3-RR (same clone as 83546-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using MBOAT7 Recombinant antibody, PBS Only (83546-3-PBS)

    IF Staining of HuH-7 using 83546-3-PBS

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-PBS, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using MBOAT7 Recombinant monoclonal antibody (83546-3-RR)

    FC experiment of A549 using 83546-3-RR (same clone as 83546-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83546-3-RR

    Affinity and Kinetic Characterization of 83546-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

MBOAT7 Recombinant monoclonal antibody, PBS Only

MBOAT7 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83546-3-PBS
Clone No.240505E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

1-acylglycerophosphatidylinositol O-acyltransferase, 240505E8, BB1, Bladder and breast carcinoma-overexpressed gene 1 protein, EC:2.3.1.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83546-3-PBS targets MBOAT7 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24466

Product name: Recombinant human MBOAT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 284-344 aa of BC006309

Sequence: SSPEKAASLEYDYETIRNIDCYSTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPARSYVLR

相同性解析による交差性が予測される生物種
Full Name membrane bound O-acyltransferase domain containing 7
Calculated molecular weight 472 aa, 53 kDa
GenBank accession numberBC006309
Gene Symbol MBOAT7
Gene ID (NCBI) 79143
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96N66
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.

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