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- KD/KO Validated
MBD2 Polyclonal antibody
MBD2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55200-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, human brain tissue, A431 cells, HEK-293 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
55200-1-AP targets MBD2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | methyl-CpG binding domain protein 2 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43-45 kDa |
| GenBank accession number | NM_003927 |
| Gene symbol | MBD2 |
| Gene ID (NCBI) | 8932 |
| RRID | AB_10852963 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MBD2 antibody 55200-1-AP | Download protocol |
| IHC protocol for MBD2 antibody 55200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
| ||
Exp Cell Res Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.
| ||
Genet Test Mol Biomarkers The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas. | ||
Gene Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Manfred (Verified Customer) (05-09-2022) | The antibody worked perfectly without any unspecific bands
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