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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAX Recombinant monoclonal antibody (82958-6-RR)

    WB analysis using 82958-6-RR (same clone as 82958-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-6-RR (MAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MAX Recombinant monoclonal antibody (82958-6-RR)

    IF Staining of PC-3 using 82958-6-RR (same clone as 82958-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MAX antibody (82958-6-RR, Clone: 230215D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

  • ChIP experiment of NCCIT cells using MAX Recombinant monoclonal antibody (82958-6-RR)

    ChIP experiment of NCCIT using 82958-6-RR (same clone as 82958-6-PBS)

    Chromatin was prepared from NCCIT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of MAX (82958-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82958-6-RR

    Affinity and Kinetic Characterization of 82958-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-6-RR against Human MAX were performed. The affinity constant is 0.257 nM.

MAX Recombinant monoclonal antibody, PBS Only

MAX Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA, ChIP-qPCR
Cat No. 82958-6-PBS
Clone No.230215D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Synonyms

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82958-6-PBS targets MAX in WB, IF/ICC, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0680

Product name: Recombinant human MAX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC003525

Sequence: MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

相同性解析による交差性が予測される生物種
Full Name MYC associated factor X
Calculated molecular weight 18 kDa
Observed molecular weight22 kDa
GenBank accession numberBC003525
Gene Symbol MAX
Gene ID (NCBI) 4149
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61244
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

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